This study examined the activity of n-hexane subfraction Plectranthus scutellarioides on normal and propylthiourasil induced hyperlipidemic rats. Propylthiourasil and high fat diet were used to induced hyperlipidemic, and also given Simvastatin 1 mg/kg body weight of rats. Fraction n-hexane of Plectranthus scutellarioides was separated by using Vacum Liquid Chromatography method (VLC) using percentage gradient eluent of n-hexane : ethyl acetat (100:0 ; 90:10 ; 80:20 ; 70:30 ; 60:40 ; 50:50 ; 40:60 ; 30:70 ; 20:80 ; 10:90 ; 0:100) and 100% ethanol. The result of separation is obtained by five subfraction (SF a, SF b, SF d, SF e, and SF f) and 3 (SF b, SF c, and SF d) of 5 subtractions were done testing with oral administration for 2 weeks. The result show that SF b provides the best results in lowering cholesterol total levels, trygliserid, LDL-C, and VLDL-C. SF c provide the best result to incrase HDL-C level.