Noni´s nanoemulsion preserves the organic functions in the presence of intestinal ischemia and reperfusion

Giovanna Dantas Fulco., Irami Araújo-Neto., Maria Helena Wanderley Costa Dantas, Tarciso Bruno Montenegro Sampaio., Amália Cinthia Meneses do Rêgoand Irami Araújo-Filho

Background: The study evaluated nanostructured extract Morinda citrifolia L. (Noni) conveyed in enteral form, in an experimental model of intestinal ischemia/reperfusion (I/R), as well as in the prevention of bacterial translocation.
Purpose: Observe the protective and repairing effect of Noni's nanoemulsified extract in the presence of induced bacterial translocation, by an experimental model of intestinal ischemia/reperfusion.
Methods and Findings: The study consisted of 2 groups of 7 animals, where the Controls were treated with 0.9% saline solution (I/R + S) and Experimental group (I/R + Noni) treated with 5 mg/mL/Kg Noni nanoemulsion, orally by gavage 12h and 2h before the experiment. In the groups, the superior mesenteric artery was occluded with a vascular micro clamp and the laparotomy reopened 60min. after for pull back the clamp. Treatment response was assessed by blood count, inflammatory markers and biochemical dosages, including a sample of the terminal ileumand hepatic of each animal was harvested, fixed in formalin 10% and included in paraffin and stained with hematoxylin-eosin for morphometric measurement. Measurement of TNF-α, IL-1β, IL-6, and IL-10 was done. One gram of spleen, liver, and mesenteric lymph nodes were harvested for culture by selective means for Gram (-) and Gram (+) bacteria. ANOVA and the post-hoc Tukey and Student´s t test were used, considering p<0,05 as significant. Conclusion: Noni´s nanoemulsion positively influenced the organic reactions in the presence of intestinal ischemia/reperfusion, reducing the production of proinflammatory cytokines, bacterial translocation, preventing tissue injury and attenuating the systemic inflammatory response against the experimental model used.

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