Effect of temperature of drying and concentration of ethyl alcohol on hypoglycemic effect of dry extract of the bilberry of the general

Author: 
Kh.I. Kurylo., Klishch I.M., Chernyashova V.V ., Volska A.S and , Stetsyuk I. O.

Introductione: the study of the effect of drying temperature and the concentration of ethyl alcohol on the hypoglycemic effect of dry bilberry extract on the model of oral glucose tolerance test on rats.
Aim of The Study: The purpose of this study was to study the effect of drying temperature and the concentration of ethyl alcohol on the hypoglycemic effect of dry bilberry extract on the model of oral glucose tolerant test on rats. Oral Glucose Tolerance Test (OTTG) is a method of studying neurohormonal regulation of glycemia due to its change in the loading process.
Materials and Methods: The object of research is dry bilberry extract (Vaccinium myrtillus L.). Experiments were performed on white non-linear male rats weighing 175 ± 20 g with normal carbohydrate homeostasis, which was assessed by basal glycemia and glucose tolerance in the model of oral glucose tolerance test. The intact control group received-distilled water. The animal is randomly assigned to the groups (n = 6): 1-intact animals, control; 2-animals of control pathology receiving an intragastric 40% glucose solution at a rate of 3 g / kg body weight; 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13-animals fed a dry bilberry extract at a dose of 50 mg / kg at different drying temperatures (500, 700, 900, 1100, 1300, 1400) pre-dried.
At the next stage of the study, the hypoglycemic effect of blueberries was estimated at a variable concentration of ethyl alcohol. Experiments were performed on white nonlinear male rats weighing 160 ± 20 g, with normal carbohydrate homeostasis, which was assessed by basal glycemia and glucose tolerance in the model of oral glucose tolerance test. The group of intact animals under the same scheme received-distilled water. The animal is randomly assigned to the groups (n = 6): 1-intact animals, control; 2-animals of control pathology receiving an intragastric 40% glucose solution at a rate of 3 g / kg body weight; 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13-animals that were injected with dry extracts of the goat and blueberries in a dose of 50 mg / kg with a different content of ethyl alcohol (0%, 10%, 20 %, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 96% respectively) of pre-dried.
Results: So, the most pronounced hypoglycemic effect of dry bilberry extracts of the usual dose of 50 mg / kg, which was extracted with 60 % ethyl alcohol and manifested at a drying temperature of 50 ° C.
Conclusion: Intragastric administration of glucose at a dose of 3 g / kg led to an increase in glucose levels at 30, 60, 90, 120, 180 min in all groups of animals, compared with the control group of animals. It was found that dry bilberry extract in a dose of 50 mg / kg dried at a drying temperature of 50 ° C and the most optimal concentration of alcoholic alcohol-60 % for extract of bilberry extracted, showed a higher hypoglycemic activity compared with other experimental groups. The obtained data testify to expediency of further experimental researches with the purpose of creation of the antidiabetic preparation.

Page: 
346-350
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