Antimicrobial susceptibilities of pneumococcal bacteria isolated from adults patients in a certain hospital in indonesia

Risrina Nur Ekawati., Tina Rostinawati and Anas Subarnas

Respiratory tract infections are serious prevalent infectious diseases which cause increase of morbidity and mortality worldwide including in Indonesia. Antibiotics used to treat these infections give effective response, but the problem emerges concerning the increasing prevalence of antibacterial resistance because of irrational use of antibiotics. This study was aimed to identify strains of bacteria isolated from respiratory tract infectious patients of adults in one of the hospitals in West Java of Indonesia and determine susceptibility of bacteria to some antibiotics. Identification of bacteria was done based on the morphological examination and gram staining test, followed up by the 16S rRNA PCR-Sequensing method for a final confirmation of the strains of bacteria. Susceptibility of bacteria was tested by agar diffusion method, and the data were compared with the related data in reference book of Clinical Laboratory Standards Institute (CLSI). The results indicated that nine strains of bacteria were identified from ten sputum samples and most were gram negative bacteria, Citrobacter kosheri, Enterobacter Sp., Pseudomonas aeruginosa, Acinetobacter bereziniae, Klebsiella Varicola, Stenotrophomonas pavanii, and Acinetobacter baumanii, and two strains were gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis. Most of the bacteria were resistant to amoxicillin and cefadroxil, some were resistant and intermediate to ceftriaxone and cefotaxime, some were resistant and susceptible to trimethoprim, and most were susceptible to sulfamethoxazole. This study concluded that the bacteria identified had variation in susceptibility to antibiotics tested, where most were resistant to β-lactam antibiotics and were susceptible and intermediate to sulfamethoxazole.

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